Duval was allegedly driving a Ford Explorer on the Hana Highway on May 29 with her sister when the vehicle plunged 200 metres off a cliff and killing Anastasia.
She was arrested and jailed on a second-degree murder charge, accused of deliberately causing her sister's death after the pair reportedly had an argument, with witnesses reporting seeing the passenger pulling the driver’s hair, officials alleged, according to the Associated Press.
However, a judge later released Duval, saying their no probable cause for a murder charge.
“We cross-examined them. They presented their evidence and the judge essentially found that there was not enough evidence to sustain the charge,” Alexandria’s attorney, Todd Eddins, reportedly said at the time.
Eddins is yet to comment on the new charges, but previously said the pair, who were well known yoga teachers, had an “exceptionally close bond”.
“The sisters had an exceptionally close bond even by identical twin standards,” Eddins told People.
“This is a devastating, heart-shattering tragedy for Alison and her family,” Eddins said in June. “We will explore all avenues to combat a charge that, in our view, is extreme and cold-hearted.”
It remains unclear what new evidence lead to Duval’s most recent arrest.